| BIT 295 | Special Topics in Biotechnology | 1-3 F,S,Sum |
| Offered as needed to present materials not normally available in regular course offerings or for offering of new courses on a trial basis. |
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| BIT 410 | Manipulation of Recombinant DNA | 4(2-5-0) F,S |
| Preq: BIO 183 or ZO/BIO 160 and CH 223 with a C- or better |
| Introduction to molecular biology and protein chemistry. Theory behind laboratory techniques and overview of cloning strategies starting from nucleic acid or protein sequence data. Laboratory sessions involve subcloning, preparation of competent cells, transformation, screening recombinant DNA by colony hybridization and PCR, SDS-PAGE of recombinant protein, affinity purification, and western blots. |
| Course Offerings: fall sprg | |
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| BIT 461 | Sequencing cDNA Libraries | 2(2-5-0) F, Alt. yrs.(even) |
| Preq: BIT 410 or MB 409 or BCH 454 or ZO 480 |
| Basic techniques in automated DNA sequencing and robotics. Colony picking and ordering cDNA libraries, use of PCR to amplify insert DNA, and strategies for large-scale automated sequencing. Principles of database management for ordering and accessing sequencing information. Half semester course, first part. |
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| BIT 462 | Gene Expression Analysis: Microarrays | 2(2-5-0) F |
| Preq: BIT 410 or BIT 510 |
| Microarray analysis is an evolving technique with its basis in the dynamic properties of the nucleic acid hybridization. We will review current theory, techniques, instrumentation, troubleshooting, analysis tools, and advanced protocols for microarray analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment and have access to exceptional instrumentation. At the conclusion of this course, students should feel comfortable with microarray experimental design, its tools, an analysis of generated data. This is a half-semester course. Student must register for both lecture and lab sections |
| Course Offerings: fall | WolfWare Info |
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| BIT (CHE) 463 | Fermentation of Recombinant Microorganisms | 2(2-5-0) S |
| Preq: BIT 410 or BIT 810 or MB 409 or BCH 454 or ZO 480 |
| Introduction to fermentation and protein chemistry. Theory behind laboratory techniques and overview of industrial scale expression systems. Laboratory sessions involve use of microbial expression vectors, fermentation systems, and large-scale purification of recombinanat protein. Half semester course, first part. |
| Course Offerings: sprg | WolfWare Info |
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| BIT (CHE) 464 | Protein Purification | 2(2-5-0) S, Alt. yrs.(even) |
| Preq: BIT 410 or MB 409 or BCH 454 or ZO 480 |
| Comparison of several different chromatography techniques for protein purification. Construction of purification tables and SDS-and native-PAGE analysis. Cost-benefit analysis of industrial-scale procedures. Half semester course, second part. |
| Course Offerings: sprg | WolfWare Info |
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| BIT 465 | Real-time PCR Techniques | 2(2-5-0) S |
| Preq: BIT 410 or BIT 510 |
| Real time PCR is an evolving technique with its basis in the dynamic properties of the polymerase chain reaction and fluorescent detection. We will review current real-time theory, techniques, machinery, troubleshooting, tools, and advanced protocols for sequence detection including SYBR green, TaqMan, Beacons, multiplexing, and single nucleotide polymorphism analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment. At the conclusion of this course, students should feel comfortable with real-time experimental design, its tools, and analysis of generated data. This is a half-semester course. Student must register for both lecture and lab sections. |
| Course Offerings: sprg | |
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| BIT 466 | Animal Cell Culture Techniques | 2(2-5-0) S |
| Preq: BIT 410 or BIT 810 |
| Introduction to animal cell culture techniques. Aseptic technique for vertebrate cell culture, media formulation, primary cell culture, long-term maintenance of cell lines, application of molecular techniques to in vitro situations. Half semester course, second part. |
| Course Offerings: fall sprg | WolfWare Info |
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| BIT 467 | PCR and DNA Fingerprinting | 2(2-5-0) F |
| Preq: BIT 510 |
| Introduction to polymerase chain reaction. Optimization of PCR reactions and primer design for DNA sequences using DNA databases available on the web. Laboratory sections include using rapid techniques for isolating and sequencing DNA from small amounts of sample and forensic identification of individuals using isolated human hairs. Credit is not allowed for both BIT 467 and BIT 567. |
| Course Offerings: fall | WolfWare Info |
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| BIT 468 | Genome Mapping | 2(2-5-0) S |
| Preq: BIT 410 or BIT 510 or BCH 454 |
| Students will be introduced to basic techniques in genetic and physical mapping. The principles of DNA marker development, marker detection, genetic and physical mapping and DNA sequencing will be addressed from a practical view with an emphasis onagricultural applications. This is a half semester course. Student must register for both lecture and lab sections. |
| Course Offerings: sprg | |
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| BIT 470 | Advanced Animal Cell Culture: Bioreactor Culture | 2(2-4-0) S |
| Preq: BIT 466 or BIT 566 or PO 566 |
| Principles of scaling animal cell seed-stock from frozen storage to three liter culture. Students will learn to assemble and operate a three-liter bioreactor to produce antibodies, as well as assess final product quantity using antibody techniques. This is a half-semester course. |
| Course Offerings: sprg | |
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| BIT (PB) 481 | Plant Tissue Culture and Transformation | 2(2-5-0) S, Alt. yrs.(odd) |
| Preq: BIT 410 or MB 409 or BCH 454 or ZO 480 |
| Basic techniques in plant tissue culture and transformation. Empirical approaches to techniques in plant tissue culture, designing transgenes for expression in specific plant cell organelles and tissues, use of reporter genes to optimize transformation, and troubleshooting transformation. Laboratory session provide hands-on experience with plant tissue culture and transformation. Use of reporter genes, fluorescence microscopy and digital imaging. Half semester course, first part. |
| Course Offerings: sprg | WolfWare Info |
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| BIT 492 | External Learning Experience | 1-6 F,S,Sum |
| Preq: BIT 410 |
| A learning experience in the area of biotechnology within an academic framework that utilizes facilities and resources which are external to the campus. Contact and arrangements with prospective employers must be initiated by student and approved by a faculty adviser, the prospective employer, and the departmental teaching coordinator prior to the experience. Project must be approved by the Academic Coordinator or Program Director of the Biotechnology Program. |
| Course Offerings: fall sum1 | |
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| BIT 493 | Special Problems in Biotechnology | 1-6 F,S,Sum |
| Preq: BIT 410 |
| A learning experience within an academic framework that utilizes campus facilities and resources. Contact and arrangements with prospective mentor(s) must be initiated by student and approved by a faculty adviser, the prospective mentor, and the departmental teaching coordinator prior to the experience. Project must be approved by the Academic Coordinator of Program Director of the Biotechnology Program. |
| Course Offerings: fall sum1 sprg | |
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| BIT 495 | Special Topics in Biotechnology | 1-3 F,S,Sum |
| Preq: BIT 410 |
| Offered as needed to present materials not normally available in regular course offerings or for offering of new courses on a trial basis. |
| Course Offerings: fall sprg | |
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| BIT 501 | Ethical Issues in Biotechnology | 1(1-0-0) S |
| Students investigate and discuss current controversial issues in biotechnology. This course emphasizes thinking about new technologies in a rational and thoughtful way. |
| Course Offerings: sprg | |
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| BIT 510 | Core Technologies in Molecular and Cellular Biology | 4(2-5-0) F,S |
| Preq: Equivalent of CH 223 and MB 351 or GN 411 |
| Basic technologies of recombinant DNA procedures, gene expression, isolation and identification of nucleic acids and proteins. |
| Course Offerings: fall sprg | WolfWare Info |
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| BIT 562 | Gene Expression: Microarrays | 2(2-5-0) F |
| Preq: BIT 410 or BIT 510 |
| Microarray analysis is an evolving technique with its basis in the dynamic properties of the nucleic acid hybridization. We will review current theory, techniques, instrumentation, troubleshooting, analysis tools, and advanced protocols for microarray analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment and have access to exceptional instrumentation. At the conclusion of this course, students should feel comfortable with microarray experimental design, its tools, an analysis of generated data. This is a half-semester course. Student must register for both lecture and lab sections |
| Course Offerings: fall | WolfWare Info |
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| BIT 563 | Fermentation of Recombinant Microorganisms | 2(2-5-0) S |
| Preq: BIT 810 or BIT 410 |
| Introduction to fermentation and protein chemistry. Theory behind laboratory techniques and overview of industrial scale expression systems. Laboratory session sinvolve use of microbial expression vectors, fermentation systems, and large-scale purification of recombinant protein. Half semester course, first part. |
| Course Offerings: sprg | |
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| BIT 564 | Protein Purification | 2(2-5-0) S, Alt. yrs.(even) |
| Preq: BIT 410 or MB 409 or BCH 454 or ZO 480 |
| Comparison of several different chromatography techniques for protein purification. Construction of purification tables and SDS-and native-PAGE analysis. Cost-benefit analysis of industrial-scale procedures. Half semester course, second part. |
| Course Offerings: sprg | |
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| BIT 565 | Real-time PCR Techniques | 2(2-5-0) S |
| Preq: BIT 410 or BIT 510 |
| Real time PCR is an evolving technique with its basis in the dynamic properties of the polymerase chain reaction and fluorescent detection. We will review current real-time theory, techniques, machinery, troubleshooting, tools, and advanced protocols for sequence detection including SYBR green, TaqMan, Beacons, multiplexing, and single nucleotide polymorphism analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment. At the conclusion of this course, students should feel comfortable with real-time experimental design, its tools, and analysis of generated data. This is a half-semester course. |
| Course Offerings: sprg | |
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| BIT (PO) 566 | Animal Cell Culture Techniques | 2(2-5-0) S |
| Preq: BIT 410 or BIT 810 |
| Introduction to animal cell culture techniques. Aseptic technique for vertebrate cell culture, media formulation, primary cell culture, long-term maintenance of cell lines, application of molecular techniques to in vitro situations. Half semester course, second part. |
| Course Offerings: fall sprg | WolfWare Info |
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| BIT 568 | Genome Mapping | 2(2-5-0) S |
| Preq: BIT 410 or BIT 510 or BCH 454 |
| Basic techniques in genetic and physical mapping. The principles of DNA marker development, marker detection, genetic and physical mapping and DNA sequencing will be addressed from a practical view with an emphasis on agricultural applications. This is a half semester course. Student must register for both the lecture and the lab. |
| Course Offerings: sprg | |
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| BIT 569 | RNA Purification and Analysis | 2(2-5-0) F |
| Preq: BIT 410 or BIT 510 |
| Laboratory-intensive course covering techniques in RNA purification and analysis including: isolation of quality RNA; quantification by gel and spectrophotometer; separation by gel electrophoresis; reverse transcription PCR; and Northern blotting using non-radioactive labeling and detection by chemi-luminescense. Half semester course. |
| Course Offerings: fall | WolfWare Info |
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| BIT 570 | Advanced Animal Cell Culture: Bioreactor Culture | 2(2-4-0) S |
| Preq: BIT 466 or BIT 566 or PO 566 |
| Principles of scaling animal cell seed-stock from frozen storage to three liter culture. Students will learn to assemble and operate a three-liter bioreactor to produce antibodies, as well as assess final product quantity using antibody techniques. This is a half-semester course. Students must register for both lecture and lab. |
| Course Offerings: sprg | |
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| BIT 595 | Special Topics | 1-3 F,S,Sum |
| Preq: BIT 410 or BIT 810 |
| Offered as needed to present materials not normally available in regular course offerings or for offering of new courses on a trial basis. |
| Course Offerings: fall sprg | |
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| BIT 815 | Advanced Special Topics | 3-6 Sum |
| Preq: BIT 810 |
| Intensive three-week or six-week courses in advanced technologies such as DNA sequencing, downstream processing, immunological techniques, construction of c-DNA libraries, mammalian embryo manipulation, plant transformation, bioreactor design, cloning in gram positive bacteria, electron microscopy or techniques in yeast molecular biology. |
| Course Offerings: fall sprg | |